DNA

Part:BBa_K241001:Design

Designed by: John Heil   Group: iGEM09_Waterloo   (2009-10-19)

phiC31 attB TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the wild type phiC31 attB site found in Streptomyces. It's minimal length was defined by Groth et al. in 2000.

Groth, A. C., Olivares, E. C., Thyagarajan, B. & Calos, M. P. (2000). A phage integrase directs efficient site-specific integration in human cells. Proc Natl Acad Sci U S A 97, 5995-6000.

Source

synthetic

References